Chemically modified amino acids enable the evolution of fed-batch processes from multiple feeds to single feed strategies

| October 22, 2018

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Cell culture optimization is critical to good biomanufacturing. In particular fed-batch cultures require optimization of feed strategies to ensure that cultures are not becoming nutrient depleted, to extend the growth phase and increase product concentration. There are several growth factors, amino acids and supplements that are used in cell culture feeds. While amino acids are one of the most popular choices, they can also be challenging because they are difficult to dissolve at high concentrations. This low solubility particularly in high concentrations necessitates that these amino acids be solubilized in separate feeds at basic pH. This can cause pH spikes, precipitation and early death when added to the bioreactor. One of the most challenging amino acids has been L-Tyrosine.

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Sirrus

Sirrus develops innovative monomer platforms that serve as the foundation for the next generation of adhesive, sealant, coating, ink and plastic solutions. The company’s team of scientists and engineers collaborates with customers across a wide range of industries on chemical formulations that enhance efficiency, reduce energy consumption and costs, and improve performance. Our products are game-changing 1,1-disubstituted alkene-based chemistry that allows products formulated with the technology to cure ambiently and bond in minutes without energy input or solvent; also enables: • reliable, flexible bonding of dissimilar materials • superior strength, chemical and temperature resistance, and optical clarity • low odor • no blooming Diverse applications for consumer products, glass, automotive, electronics, millwork, packaging and healthcare applications

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